A survey was carried out in Bulgaria to characterize PPV strains. The universal 5BMAb and two strain-specific monoclonal antibodies (MAb4DG5 for PPV-D and MAbAL for PPV-M) were used in DASI-ELISA test for virus detection and strain differentiation. PPV infection level in different fruit species was as follows: plum (62 percent), apricot (23 percent ), peach (19 percent) and cherry (0 percent). Only PPV-M strain was identified in peaches and apricots. PPV-M and PPV-D incidence in plum was respectively 88 percent and 5 percent. Mixed infection (M+D) in plum was 6 percent. In the Kustendil region, infection rate of PPV-M strain was 75 percent, PPV-D of 12 percent and M+D of 13 percent. PPV-M confirms its dominant and endemic presence in Bulgaria.